President's DNA Initiative
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Advancing Justice Through DNA Technology
DNA Analyst Training
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Course Content

Home > Course Introduction > Intro to the Academy > Course Content

The course content with notes is given below.

Course Content

Course Introduction

Core Content

  • Subject 1: Evidence and DNA
    • Module 1: Evidence
    • Module 2: DNA
  • Successful completion required by all students, except those with a proven competency in the area, such as a serologist being re-trained to become a DNA analyst.
  • Subject 2: Forensic Biology
    • Module 1: Laboratory orientation
    • Module 2: Testing of body fluids and tissues
  • Successful completion required by all students, except those with a proven competency in the area, such as a serologist being re-trained to become a DNA analyst.
  • Successful completion of practical exercises according to the Individual Training Plan required.
  • Subject 3: DNA Extraction and quantitation
    • Module 1: DNA Analysis considerations
    • Module 2: Organic extraction
    • Module 3: Chelex 100® extraction
    • Module 4: Other extraction techniques
    • Module 5: Quantitation
  • Successful completion required by all students.
  • Note: The laboratory may elect to focus on the extraction techniques used in their laboratories. However, it is strongly recommended that the student at least reads through the content of the modules covering the techniques not used in their laboratory.
  • Successful completion of practical exercises according to the Individual Training Plan required.
  • Subject 4: DNA Amplification
    • Module 1: Overview
    • Module 2: Locus selection
    • Module 3: Multiplexing
    • Module 4: Contamination
  • Successful completion required by all students
  • Successful completion of practical exercises according to the Individual Training Plan. 
  • Note that the practical exercises for Subject 4 will be taken along with the exercises for Subjects 5 and 6.
  • Subject 5: Amplified DNA Product Separation
    • Module 1: Overview
    • Module 2: Capillary electrophoresis
    • Module 3: Sampling preparation & handling
  • Successful completion required by all students
  • Successful completion of practical exercises according to the Individual Training Plan. 
  • Note that the practical exercises for Subject 5 will be taken along with the exercises for Subjects 4 and 6.
  • Subject 6: STR Data Analysis & Interpretation
    • Module 1: Software
    • Module 2: Data troubleshooting
    • Module 3: Data interpretation& allele calls
  • Successful completion required by all students
  • Successful completion of practical exercises according to the Individual Training Plan.
  • Note that the practical exercises for Subject 6 will be taken along with the exercises for Subjects 4 and 5.
  • Subject 7: Population Genetics & Statistics
    • Module 1: Population theory
    • Module 2: Statistics
  • Successful completion required by all students
  • Note that the standard of the test is higher than for the other modules because this topic as required by the QA standards (Standard 5.3.1).  Also it is possible to take this class for credit – contact NFSTC for more information.
  • Successful completion of practical exercises according to the Individual Training Plan
  • Subject 8: Communicating Results
    • Module 1: Communication skills
    • Module 2: Report writing
    • Module 3: Courtroom testimony
  • Successful completion required by all students
  • Successful completion of practical exercises (mock trial) according to the Individual Training Plan.
  • Subject 9: Other DNA Markers & Technologies
    • Module 1: Other nuclear DNA markers & technologies
    • Module 2: Non-nuclear DNA markers
    • Module 3: Techniques
  • Successful completion required by all students

Course Resources

  • National Quality Assurance Standards
  • CODIS
  • Safety
  • Professionalism and ethics
  • Literature
  • This module contains a range of literature references of interest.  There are separate literature citations in each module. 

Laboratory Manual

  • The manual provides a comprehensive guide to the exercises required to demonstrate practical competency.  The exercises are aligned with the material in the knowledge base and with the recommendations of the SWGDAM Training Guidelines.

Train the Trainer

  • The Technical Leader (and Training Manager) should work through this module before implementation of the training.

Foundation Sciences

  • Biochemistry
  • Molecular Biology
  • Genetics
  • In the case of students who do not meet the national QA standards requirements for one or more of the foundation sciences, arrangements can be made to take the class(es) for credit (contact the NFSTC by e-mail). These may also serve as non-credit refresher courses.

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