President's DNA Initiative
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DNA Analyst Training
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Collection Techniques

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The importance of avoiding cross contamination can not be overemphasized. The investigator performing the collection must ensure tools are clean and/or sterilized and that gloves are changed between handling each sample.

Collection methods differ depending on the type of evidence and the substrate upon which it is found. It is preferable to collect evidence in its original state. If the evidence is fragile or can easily be lost, the entire object should be collected and packaged, if size and circumstances permit.

Some laboratories recommend the submission of substrate controls. Substrate controls are clean samples of the collection materials or unstained portions of the material the biological evidence is deposited on. The laboratories can use these to troubleshoot contamination, Polymerase Chain Reaction (PCR) inhibition, or interference with fluorescence.

The investigator should consult the local forensic laboratory and refer to the department standard operating procedures regarding collection and preservation of biological evidence.

Procedures for Evidence Collection

Blood & Other Body Fluids

Type of Collection

Procedure

Cuttings

Removal of a section of the item containing the stain using a sterile or clean cutting device.

Wet Absorption

A sterile swab, gauze pad, or threads are slightly moistened with sterile distilled water. An effort should be made to concentrate the stain in a localized portion of the swab or pad. For example, when using a swab, the stain should be concentrated on the tip. The collection medium is concentrated into the stain and allowed to air dry. Some laboratories recommend following the first moistened swabbing with a second dry swabbing to ensure thorough sample collection. Both swabs are retained and submitted for analysis.

Scraping Method

Using a clean razor blade or scalpel, the sample is scraped into a clean piece of paper that can be folded and packaged in a paper envelope or other appropriate packaging.

Lifting with Tape

For dried blood stains on a non-absorbent surface, fingerprint lifting tape may be placed over the stain and lifted off. The stain is transferred to the adhesive side of the tape, which may then be secured on a clear piece of acetate for submission to the laboratory.

Hair & Fiber Collection

Type of Collection

Procedure

Visual Collection

On some surfaces, hairs and fibers can be seen with the naked eye. Using clean forceps and trace paper, the sample can be removed from the surface and placed into a clean piece of paper that can be folded and packaged in a paper envelope or other appropriate packaging.

Tape Lifting

Water or methanol soluble tapes are available for the collection of trace hair and fiber evidence. The tape is applied to the location of the suspected sample, removed, and packaged.

Vacuuming Method

The area where the suspected samples are located are vacuumed up and caught in a filtered trap attached to the vacuum. These samples are packaged in clean trace paper for submission to the laboratory. Vacuuming is the least desirable collection method because there is a risk of cross contamination if the equipment is not properly cleaned between each use.

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© 2007 NFSTC Science Serving Justice®
NOTE TO USERS: The President’s DNA Initiative DNA Analyst Training program and assessment were completed and published in 2005, in cooperation with the National Institute of Justice. The science and techniques in the program are sound and proven, however, program content has not been updated to include tools and technologies developed and in use after 2005, including many kits and robots. Assessment questions address only content delivered in this program and may not contain the full range of tools in use in your laboratory.