Introduction
DNA testing is a relatively recent technological advance in the field of forensic biology. The technology has a remarkable power to discern genetic differences, pretty well to the point of individualization. Coupled with the development of increasingly sensitive methods, DNA testing is now an essential part of the crime laboratory’s armamentarium in the investigation of crimes against the person.
Objectives
Upon successful completion of this unit of instruction, the student shall be able to do the following:
- Understand the history of forensic science and the use of restriction fragment length polymorphisms (RFLP)
- Understand the method in which DNA profiles are developed, including the use of oligonucleotides as probes
- Understand the Disadvantages of RFLP Analysis
- Understand the concept of short tandem repeats (STR) testing including multiplexing and the ability to label nucleotides with fluorescent tags
- Describe the history of forensic DNA testing
- Describe the advantages of STR typing over earlier methods
- Describe the sequence of steps involved in DNA typing
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