Probes will bind specifically to complementary VNTR fragments. Unbound and non-specifically bound probe is removed using a washing process. The RFLP profile is then visualized by exposing the membrane to film or through the use of equipment, such as the Kodak imaging station.
Click here to visit the Kodak website to read more about the KODAK Image Station 4000R Digital Imaging System.
The technique originally developed by Jeffreys used multi-locus probes which could hybridize to several VNTR sites.
Alternatively, profiles can be developed by using a single probe process. The membrane containing the immobilized fragments is treated with one probe and visualized. This single probe is then removed and another probe is applied to the membrane. This was the most common method used in the United States and the process generally consisted of four to six probes. Single locus probe procedures are easier to interpret and allow for simplified court presentation.
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