President's DNA Initiative
-
Advancing Justice Through DNA Technology
DNA Analyst Training
Home
Glossary
Index
Site Map
User Guide
Course Introduction
01: Evidence & DNA
02: Forensic Biology
03: DNA Extraction & Quantitation
DNA Analysis Considerations
Introduction
Substrates
Inhibitors
Overcoming Inhibition
Works Cited & Online Links
Organic Extraction
Introduction
Organic Extraction Process
Solubilization of Stain Components
Denaturation and Hydrolysis of Proteins
Removal of Denatured Proteins
DNA Purification
Techniques
Differential Method
Works Cited & Online Links
Chelex
®
100 Extraction
Introduction
Extraction Process
Advantages and Disadvantages
Non-Differential
Differential
Works Cited & Online Links
Other Extraction Techniques
Introduction
Modifications
Microcentrifuge Tubes with Spin Baskets
Centrifugal Filter Units
Other Techniques
Spin Column
Silica Beads
Magnetic Beads
FTA
®
Technology
Works Cited & Online Links
Quantitation
Introduction
Overview
Yield Gels
Absorption Spectrophotometry
Fluorometry
Slot Blot
AluQuant
Quantitative PCR
SYBR
®
Green Detection Method
Fluorescent Resonance Energy Transfer (FRET)
Data Analysis for Quantitative PCR
Summary of Advantages and Disadvantages
Works Cited & Online Links
04: DNA Amplification
05: Amplified DNA Product Separation
06: STR Data Analysis & Interpretation
07: Population Genetics & Statistics
08: Communicating Results
09: Other DNA Markers & Technologies
Course Resources
Laboratory Training Manual
DNA Extraction & Quantitation
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