President's DNA Initiative
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Advancing Justice Through DNA Technology

DNA Analyst Training

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Introduction

Home > DNA Extraction & Quantitation > Quantitation > Introduction

Quantitation refers to determining the quantity or concentration of extracted DNA in a biological sample. It is required by the quality assurance standards (QAS) as a step in the analysis of evidence samples.

Objectives

Upon successful completion of this unit of instruction, the student shall be able to:

• List the reasons quantitation is necessary
• List the advantages and disadvantages of the principal DNA quantitation methods
• Understand the basic underlying chemistry involved in several quantitation methods including slot blots, luminescent reactions, and quantitative PCR
• Discuss proprietary kits available for DNA quantitation commonly used in forensic laboratories
• Perform calculations and dilutions to target specific amounts of DNA suitable for PCR

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