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SYBR^® Green Detection Method

Home > DNA Extraction & Quantitation > Quantitation > Quantitative PCR > SYBR^® Green Detection Method

The Quantitative PCR instrument consists of a thermal cycler housed together with a digital fluorometer detector. The principle behind the method is that as the PCR amplification process progresses, there is an increase in fluorescence from SYBR^® Green dye. As the SYBR^® Green dye binds to double-stranded amplicons, it undergoes a conformational change and emits fluorescence at a greater intensity.07 For forensic DNA analysis the most common SYBR^® Green method is Alu-based, which targets human specific Alu sequences.08

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