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DNA Template

Home > DNA Amplification > Overview > Thermal Cyclers > DNA Template

The concentration of template in a sample can affect the success of PCR amplification in a variety of ways. Too much template promotes nonspecific binding of primers to secondary sites or changes the pH of the reaction mix. Too little template can result in poor yields and/or preferential amplification, especially if the template is degraded. It should be noted that very low template concentration (e.g. 10 copies) can be sufficient for successful PCR amplification.

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NOTE TO USERS: The President’s DNA Initiative DNA Analyst Training program and assessment were completed and published in 2005, in cooperation with the National Institute of Justice. The science and techniques in the program are sound and proven, however, program content has not been updated to include tools and technologies developed and in use after 2005, including many kits and robots. Assessment questions address only content delivered in this program and may not contain the full range of tools in use in your laboratory.