- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- Overview
- Introduction
- Amplification
- Primer Design
- Primer Length
- Primer Melting Temperature
- Primer Annealing Temperature
- Values for Tm
- Maximum 3′ Stability
- GC Content Percentage
- No Secondary Structures
- No Self-complementarity
- No Complementarity To Other Primers
- No Long Runs With The Same Base
- Distance Between Two Primers On Target Sequence
- Plateau Effect
- Thermal Cyclers
- Thermal Cycling Parameter and Optimization
- Works Cited & Online Links
- Locus Selection
- Multiplexing
- Contamination
- Overview
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- 07: Population Genetics & Statistics
- 08: Communicating Results
- 09: Other DNA Markers & Technologies
- Course Resources
- Laboratory Training Manual
President's DNA Initiative
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Advancing Justice Through DNA Technology
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Advancing Justice Through DNA Technology
DNA Analyst Training
CODIS Core Loci
Home > DNA Amplification > Locus Selection > CODIS Core Loci
In 1994, the U.S. Congress passed the DNA Identification Act (Public Law No. 103-322 -1994).01 This law, in part, mandated that the FBI was responsible for the Combined DNA Index System (CODIS).
Click here to read more about CODIS in the CODIS module.
In 1996, the Department of Justice, through the FBI and the Technical Working Group on DNA Analysis Methods (TWGDAM), funded the CODIS STR Standardization Project.
The purpose of this project was to evaluate various STR loci and to establish core loci for the CODIS. Crime laboratories from Alabama, Arizona, California, Florida, Michigan, New York, North Carolina, Oregon, Texas, and Virginia, together with the Armed Forces DNA Identification Laboratory (AFDIL), the FBI, the National Institute of Standards and Technology (NIST), and the Royal Mounted Canadian Police (RCMP) participated in the study. Two manufacturers, Applied Biosystems and Promega, also participated in the STR Standardization Project.
Prior to this project, each manufacturer had forensic multiplex STR kits available:
- Applied Biosystems AmpFℓSTR® Profiler™
- Amelogenin
- CSF1PO
- D3S1358
- D5S818
- D7S820
- D13S317
- FGA
- TH01
- TPOX
- vWA
- Applied Biosystems AmpFℓSTR® Green II™
- Amelogenin
- D8S1179
- D18S51
- D21S11
- Promega PowerPlex®
- CSF1PO
- D5S818
- D7S820
- D13S317
- D16S539
- TH01
- TPOX
- vWA
