- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- Overview
- Introduction
- Amplification
- Primer Design
- Primer Length
- Primer Melting Temperature
- Primer Annealing Temperature
- Values for Tm
- Maximum 3′ Stability
- GC Content Percentage
- No Secondary Structures
- No Self-complementarity
- No Complementarity To Other Primers
- No Long Runs With The Same Base
- Distance Between Two Primers On Target Sequence
- Plateau Effect
- Thermal Cyclers
- Thermal Cycling Parameter and Optimization
- Works Cited & Online Links
- Locus Selection
- Multiplexing
- Contamination
- Overview
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- 07: Population Genetics & Statistics
- 08: Communicating Results
- 09: Other DNA Markers & Technologies
- Course Resources
- Laboratory Training Manual
President's DNA Initiative
-
Advancing Justice Through DNA Technology
-
Advancing Justice Through DNA Technology
DNA Analyst Training
Applied Biosystems
Home > DNA Amplification > Locus Selection > CODIS Core Loci > Applied Biosystems
Although other kits were available, the AmpFℓSTR® and PowerPlex® kits were the main kits used during the CODIS STR Standardization Project.
The CODIS STR Standardization Project identified thirteen core loci, together with amelogenin:
- CSF1PO
- D3S1358
- D5S818
- D7S820
- D8S1179
- D13S317
- D16S539
- D18S51
- D21S11
- FGA
- TH01
- TPOX
- vWA
The two manufacturers responded rapidly by developing new kits that included the thirteen core loci and amelogenin. The manufacturers involved the forensic science community in the beta testing of the kits, requested feedback, and responded to numerous suggested changes. The cooperation was mutually beneficial. The industry was able to develop technology that would be readily accepted by the community; the community enabled industry to build a robust technology tailored to forensic STR testing.
Applied Biosystems retooled AmpFℓSTR® Profiler™ and developed AmpFℓSTR® Profiler Plus™ and AmpFℓSTR® COfiler™.
) Applied Biosystems Profiler Plus |
) Applied Biosystems COfiler |
The loci included in these Applied Biosystems are: (highlighted loci are included in both kits)
Comparison of Applied Biosystems Kits |
|
AmpFℓSTR® Profiler Plus™ |
AmpFℓSTR® COfiler™ |
D3S1358 |
D3S1358 |
D5S818 |
|
D7S820 |
D7S820 |
D8S1179 |
D16S539 |
D13S317 |
CSF1PO |
D18S51 |
TH01 |
FGA |
TPOX |
vWA |
|
Amelogenin |
Amelogenin |
The combination of these two kits allows laboratories to test all core loci; the overlapping loci serve as a quality control measure in the testing process.
