- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- Overview
- Introduction
- Amplification
- Primer Design
- Primer Length
- Primer Melting Temperature
- Primer Annealing Temperature
- Values for Tm
- Maximum 3′ Stability
- GC Content Percentage
- No Secondary Structures
- No Self-complementarity
- No Complementarity To Other Primers
- No Long Runs With The Same Base
- Distance Between Two Primers On Target Sequence
- Plateau Effect
- Thermal Cyclers
- Thermal Cycling Parameter and Optimization
- Works Cited & Online Links
- Locus Selection
- Multiplexing
- Contamination
- Overview
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- 07: Population Genetics & Statistics
- 08: Communicating Results
- 09: Other DNA Markers & Technologies
- Course Resources
- Laboratory Training Manual
President's DNA Initiative
-
Advancing Justice Through DNA Technology
-
Advancing Justice Through DNA Technology
DNA Analyst Training
Promega
Home > DNA Amplification > Locus Selection > CODIS Core Loci > Promega
Promega continued to offer their original PowerPlex® 1.X kit and developed a second kit, PowerPlex® 2.1 (highlighted loci are included in both kits).
Comparison of Promega Kits |
|
PowerPlex®1.X |
PowerPlex®2.1 |
D5S818 |
D3S1358 |
D7S820 |
D8S1179 |
D13S317 |
D18S51 |
D16S539 |
D21S11 |
CSF1PO |
FGA |
TH01 |
TH01 |
TPOX |
TPOX |
vWA |
vWA |
|
Penta E |
|
Amelogenin |
The combination of these two PowerPlex® kits provides a degree of overlap (vWA, TPOX, THO1) and adds an additional locus, Penta E.
) Promega PowerPlex 1.1 |
) Promega PowerPlex 2.1 |
The two manufacturers use different primer sets in their kits. This can be an advantage in detecting apparent null alleles caused by primer site mutations.02 Although the occurrence of discordance between the two manufacturers’ kits is rare, it is something that analysts must consider when comparing profiles. An analyst can differentiate between a discordance event and a true exclusion between two samples.
Prior to uploading a sample, any laboratory participating in NDIS must attempt to develop results at all 13 core loci and must obtain results at 10 in order to upload the profile. Requiring a minimum of ten loci reduces the chance of adventitious hits, allowing agencies to focus their resources on more discriminating investigative leads.
