- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- Overview
- Introduction
- Amplification
- Primer Design
- Primer Length
- Primer Melting Temperature
- Primer Annealing Temperature
- Values for Tm
- Maximum 3′ Stability
- GC Content Percentage
- No Secondary Structures
- No Self-complementarity
- No Complementarity To Other Primers
- No Long Runs With The Same Base
- Distance Between Two Primers On Target Sequence
- Plateau Effect
- Thermal Cyclers
- Thermal Cycling Parameter and Optimization
- Works Cited & Online Links
- Locus Selection
- Multiplexing
- Contamination
- Overview
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- 07: Population Genetics & Statistics
- 08: Communicating Results
- 09: Other DNA Markers & Technologies
- Course Resources
- Laboratory Training Manual
President's DNA Initiative
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Advancing Justice Through DNA Technology
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Advancing Justice Through DNA Technology
DNA Analyst Training
Works Cited & Online Links
Home > DNA Amplification > Locus Selection > Works Cited & Online Links
- U.S. Congress. House. 1994. The Violent Crime Control and Law Enforcement Act of 1994. Public Law 103-322.
- Budowle, B. 2000. STR allele concordance between different primer sets: A brief summary. Profiles in DNA, Promega publication 3 (3):10–11. http://www.promega.com/profiles/303/ ProfilesinDNA_303_10.pdf.
- Sparkes, R., C. Kimpton, S. Watson, N. Oldroyd, T. Clayton, L. Barnett, and J. Arnold, et al. 1996. The validation of a 7-locus multiplex STR test for use in forensic casework. (I). Mixtures, ageing, degradation and species studies.Int J Legal Med 109 (4): 186–94.
- Sparkes, R., C. Kimpton, S. Gilbard, P. Carne, J. Andersen, N. Oldroyd, D. Thomas, A. Urquhart, and P. Gill. 1996. The validation of a 7-locus multiplex STR test for use in forensic casework. (II). Artefacts, casework studies and success rates. Int J Legal Med 109 (4): 195–204.
- Kayser, M. et al. 1997. Evaluation of Y-chromosomal STRs: A multicenter study. International Journal of Legal Medicine 110(3): 125-133, 141-149.
- Applied Biosystems. 2005. AmpFlSTR® SGM Plus® PCR Amplification Kit User’s Manual.
- Gill, P., L. Fereday, N. Morling, and P. M. Schneider. 2006. The evolution of DNA databases: Recommendations for new European STR loci. Forensic Sci Int 156 (2–3): 242–44.
- Scientific Working Group on DNA Analysis Methods (SWGDAM) Y-STR Subcommittee. 2004. Report on the current activities of the Scientific Working Group on DNA Analysis Methods Y-STR Subcommittee. Forensic Science Communications 6 (3). http://www.fbi.gov/hq/lab/fsc/backissu/july2004/standards/ 2004_03_standards03.htm.
- Kayser, M., A. Caglia, D. Corach, N. Fretwell, C. Gehrig, G. Graziosi, F. Heidorn, et al. 1997. Evaluation of Y-chromosomal STRs: A multicenter study. Int J Legal Med 110 (3): 125–33, 141–49.
Online Links
- ENFSI
http://www.enfsi.org/ - FBI Laboratory Services
http://www.fbi.gov/hq/lab/org/labchart.htm - Forensic Science Service
http://www.forensic.gov.uk/forensic_t/index.htm - Human Genome Project
http://ornl.gov/swci/techresources/Human_Genome/home.shtml - INTERPOL
http://www.interpol.int/
