- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- Overview
- Introduction
- Amplification
- Primer Design
- Primer Length
- Primer Melting Temperature
- Primer Annealing Temperature
- Values for Tm
- Maximum 3′ Stability
- GC Content Percentage
- No Secondary Structures
- No Self-complementarity
- No Complementarity To Other Primers
- No Long Runs With The Same Base
- Distance Between Two Primers On Target Sequence
- Plateau Effect
- Thermal Cyclers
- Thermal Cycling Parameter and Optimization
- Works Cited & Online Links
- Locus Selection
- Multiplexing
- Contamination
- Overview
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- 07: Population Genetics & Statistics
- 08: Communicating Results
- 09: Other DNA Markers & Technologies
- Course Resources
- Laboratory Training Manual
President's DNA Initiative
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Advancing Justice Through DNA Technology
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Advancing Justice Through DNA Technology
DNA Analyst Training
Introduction
Home > DNA Amplification > Multiplexing > Introduction
Multiplexing is the simultaneous amplification of more than one locus. This greatly improves the laboratory's casework throughput because the loci are analyzed concurrently rather than sequentially. However, for multiplexing to be effective, the loci must be capable of being analyzed on the same gel or the same capillary electrophoresis run. Factors such as technology and optimal inter-locus spacing have to be considered.
Objectives
Upon successful completion of this unit of instruction, the student shall be able to:
- Describe how multiplexing has effected the process of forensic DNA analysis.
- Describe the components, instruments and amplification kits that make multiplexing feasible.
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Explain the historical development of forensic multiplex DNA kits in the United States.
