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AmpFℓSTR® (Applied Biosystems)

Home > DNA Amplification > Multiplexing > Proprietary Kits > AmpFℓSTR® (Applied Biosystems)

Applied Biosystems was the first kit manufacturer to produce an STR multiplex kit for DNA testing.

Applied Biosystems Kit Chronology

Kit

Loci

Dye

Comments

AmpFℓSTR® Blue™
  • D3S1358
  • vWA
  • FGA

Blue 5-FAM™
  • Lower discrimination power compared to the larger multiplexes currently used in forensic laboratories
  • Important precursors in the evolution of forensic STR multiplex testing

AmpFℓSTR® Green I™
  • Amelogenin
  • THO1
  • TPOX
  • CSF1PO

Green JOE™

 

AmpFℓSTR® Profiler Plus™
  • D3S1358
  • vWA
  • FGA

Blue 5-FAM™
  • Used in combination with AmpFℓSTR® COfiler™, meets the national requirements for analyzing the 13 CODIS core loci
  • Incorporates a quality control measure by ensuring concordance of the repeated loci between the two kits (D3S1358, D7S820, and amelogenin), which can assist the laboratory in detecting tube mix-ups.
• Screen Shot of AmpFℓSTR® Profiler Plus™ Ladder
Click for larger image
  • Amelogenin
  • D8S1179
  • D21S11
  • D18S51

Green JOE™
  • D5S818
  • D13S317
  • D7S820

Yellow NED™

 

AmpFℓSTR® COfiler™
  • D3S1358
  • D16S539

Blue 5-FAM™
  • Used in combination with AmpFℓSTR® Profiler Plus™, meets the national requirements for analyzing the 13 CODIS core loci
  • Incorporates a quality control measure by ensuring concordance of the repeated loci between the two kits (D3S1358, D7S820, and amelogenin), which can assist the laboratory in detecting tube mix-ups.03, 04
• Screen Shot of AmpFℓSTR® COfiler™ Ladder
Click for larger image
  • Amelogenin
  • THO1
  • TPOX
  • CSF1PO

Green JOE™
  • D7S820

Yellow NED™

 

AmpFℓSTR® Identifiler™
  • D8S1179
  • D21S11
  • D7S820
  • CSF1PO

6-FAM™
  • Faster and less costly
  • Tests for all of the core loci with a single amplification
  • Also amplifies D2S1338 and D19S433, which increases the power of discrimination 05
• Screen Shot of AmpFℓSTR® Identifiler™ Ladder
Click for larger image
  • D3S1358
  • THO1
  • D13S317
  • D16S539
  • D2S1338

Green VIC™
  • D19S433
  • vWA
  • TPOX
  • D18S51

Yellow NED™
  • Amelogenin
  • D5S818
  • FGA

Red PET™

To establish a suitable layout for the sample run, Applied Biosystems introduced the use of non-nucleotide linkers to the following primers:  CSF1PO, D2S1338, D13S317, D16S539 and TPOX. Non-nucleotide linkers are placed between the primer and the fluorescent dye during oligonucleotide synthesis. 06, 07 This allows for reproducible typing of alleles and provides sufficient spacing between the loci in the multiplex.

Applied Biosystems introduced a Y-STR kit (AmpFℓSTR® Yfiler™) to test for the core Y-STR loci established by the Scientific Working Group on DNA Analysis Methods (SWGDAM).

Applied Biosystems AmpFℓSTR® Yfiler™ Kit

Loci

Dye

Comments
  • DYS456
  • DYS389I
  • DYS390
  • DYS389II

Blue 6-FAM™
  • Core Y-STR loci recommended by the SWGDAM Y chromosome subcommittee in 2003 included DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438,  DYS439, DYS385 (DYS385 is multi-copy)
  • Tests for 17 Y-STR loci 08
• Screen Shot of AmpFℓSTR® Yfiler™ Ladder
Click for larger image
  • DYS458
  • DYS19
  • DYS385

Green VIC™
  • DYS393
  • DYS391
  • DYS439
  • DYS635
  • DYS392

Yellow NED™
  • H4
  • DYS437
  • DYS438
  • DYS448

Red PET™

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