- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- Overview
- Introduction
- Amplification
- Primer Design
- Primer Length
- Primer Melting Temperature
- Primer Annealing Temperature
- Values for Tm
- Maximum 3′ Stability
- GC Content Percentage
- No Secondary Structures
- No Self-complementarity
- No Complementarity To Other Primers
- No Long Runs With The Same Base
- Distance Between Two Primers On Target Sequence
- Plateau Effect
- Thermal Cyclers
- Thermal Cycling Parameter and Optimization
- Works Cited & Online Links
- Locus Selection
- Multiplexing
- Contamination
- Overview
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- 07: Population Genetics & Statistics
- 08: Communicating Results
- 09: Other DNA Markers & Technologies
- Course Resources
- Laboratory Training Manual
President's DNA Initiative
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Advancing Justice Through DNA Technology
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Advancing Justice Through DNA Technology
DNA Analyst Training
Implementation of Clean Techniques
Home > DNA Amplification > Contamination > Prevention > Implementation of Clean Techniques
Implementation of clean techniques to reduce contamination is covered in Subject 2 Module 1. More information about detailed prevention practices can be found in this module.
Click here to read about detailed prevention practices in Subject 02, Module 01.
Reference and Evidentiary Sample Processing
One concern with contamination is that an individual may be falsely linked to a crime. Reference samples are generally good quality DNA samples and result in high quantities of extracted DNA. Many laboratories process samples in a way that isolates evidentiary samples from reference samples during the screening, extraction, and PCR stages. Therefore, the possibility of contaminating an evidentiary sample with reference DNA is avoided.
