- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- 07: Population Genetics & Statistics
- 08: Communicating Results
- 09: Other DNA Markers & Technologies
- Course Resources
- Laboratory Training Manual
President's DNA Initiative
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Advancing Justice Through DNA Technology
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Advancing Justice Through DNA Technology
DNA Analyst Training
FMBIO® Fluorescence Imaging Scanners
Home > Amplified DNA Product Separation > Overview > Historical Methods > FMBIO® Fluorescence Imaging Scanners
Still used in a limited number of laboratories, the FMBIO® platform provided an easy to use detection system for analysis of amplified product from multiplexed reactions.
The FMBIO® II, III, and III Plus systems are versatile flat bed scanners capable of capturing data from a range of applications. They are used after product separation on PAGs, and depend on post-electrophoretic detection methods. The FMBIO® systems are laser-based imaging systems with up to eight-color fluorescence detection and provide superior sensitivity to a slab gel with products visualized by silver staining.
Click here to view an animation on fluorescence.
The samples are electrophoresed through a PAG in a tank apparatus, the plates are cleaned, placed directly on the scanner, and the software detects the fluorescently labeled bands (amplified product) on the gel. The bands are detected after electrophoresis using the scanning laser. These systems are sensitive, display the results, and conduct the analysis. The FMBIO® systems have a laser, filters, and two sets of detectors for the simultaneous capture of two wavelengths. The electrophoregrams are stored electronically.
Click here to view a table presenting instruments, solid-state laser, and kist used with the instruments.
