Preparation of amplified products for electrokinetic injection is extremely important. The manner in which the samples are denatured and the resuspension solution used can affect both the sensitivity and the sample peak resolution. Proper sample preparation for capillary electrophoresis is a significant step in the analysis of forensic specimens.
Upon successful completion of this unit of instruction, the student shall be able to:
- Explain the denaturation process of amplified DNA products for sample injection.
- Recognize the importance of formamide quality.
- Explain the quality control measures for assessing formamide quality
- Explain the pros/cons of using water in place of formamide
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