- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- Data Troubleshooting
- Data Interpretation & Allele Calls
President's DNA Initiative
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Advancing Justice Through DNA Technology
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Advancing Justice Through DNA Technology
DNA Analyst Training
Overview
Home > STR Data Analysis & Interpretation > STR Data Analysis & Interpretation Software > Overview
Data produced in the separation and characterization of amplified DNA is displayed as:
- Peaks (capillary electrophoresis)
- Bands (slab gel electrophoresis)
The DNA fragments are sized, which includes an indirect assessment of quantity present (peak area/height or band density), and genotypes are assigned. The conversion of sized DNA fragments to genotypes is the standardization between all forensic DNA laboratories for comparing data and is essential for laboratories utilizing CODIS to compare profiles.
Click here to learn more about CODIS in Subject 10.
The steps for converting fluorescent data/peaks into allele calls are shown below with the corresponding software noted to the right of the specific steps.01
Click here to view an animation about the basic components of an electropherogram.
