Genotyper® converts GeneScan® sized peaks into genotype calls using predefined macros, providing defined results. Genotyper® uses the tabular data from GeneScan® to make allele calls using the first ladder recognized by the program. The two main manufacturer macros for the proprietary kits are Kazam for analysis of AmpFℓSTR® amplification kits and PowerTyper™ for Promega products.
These macros do three things:
- Calculate the bin offsets (alleles) based on the tabular data for sizing
- Filter stutter as defined in the macro
- Assign the number(s) that represent the genotypes for the profile (based on the ladder used for the sample set)
The macro is simply a step list of actions that are performed sequentially as defined when a particular template is launched. Macros are used to check the size standard by attaching the labeled sizes for confirmation; others can be written to label peaks and to set up Genotyper® tables. After assignment of allele labels, the analyst proceeds with the interpretation of the overall profile.
Click here to view Genotyper's macros window.
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