- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- Data Troubleshooting
- Data Interpretation & Allele Calls
President's DNA Initiative
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Advancing Justice Through DNA Technology
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Advancing Justice Through DNA Technology
DNA Analyst Training
Features and Procedures: Yfiler Kit
Home > STR Data Analysis & Interpretation > STR Data Analysis & Interpretation Software > GeneMapper® ID > Features and Procedures: Yfiler Kit
Features have been added in GeneMapper® ID Software v3.2 to facilitate analysis of the AmpFℓSTR® Yfiler™ PCR Amplification Kit.
Features and Procedures (v3.2) |
|
Feature/Procedure |
Description |
New feature: Allele calling parameters for new marker repeat types |
In GeneMapper ID Software v3.1, allele calling parameters were only available for markers with tetranucleotide repeat motifs. In GeneMapper® ID Software v3.2, however, allele calling parameters are available for four marker repeat types: tri-, tetra-, penta-, and hexanucleotide. The four allele calling parameters appear in the Allele tab of the Analysis Method Editor. All related analysis values are entered directly into the Tri, Tetra, Penta, or Hexa column fields, which allows for viewing of all values. |
New feature: Plus stutter filtering (for the DYS392 locus) |
To aid in interpreting genotype profiles, two new fields have been added to the Allele tab of the Analysis Method Editor specifically to filter out the DYS392 plus stutter:
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Procedure: Workaround for the DYS19 locus (-2bp filtering) |
Laboratories may choose to implement a workaround for the DYS19 locus by using the Minus A Ratio and Minus A Distance fields in the Allele tab of the Analysis Method Editor. These fields can be used to filter out the -2-bp stutter that is observed at the DYS19 locus. |
Procedure: Creating HID analysis methods for the Yfiler kit |
Use the Analysis Method Editor in GeneMapper® ID Software v3.2 to set analysis parameter values for analyzing the Yfiler kit data. |
Procedure: Creating a table setting and uploading exported haplotype(s) for searching profiles with the Yfiler™ Haplotype Database |
Using the Table Setting Editor, a table setting is created in GeneMapper® ID Sortware v3.2 to export haplotypes specifically for searching the Yfiler Haplotype Database for profile match estimation. |
Genotyper® had a feature that allowed review of the sizing standard for precision assessment. This feature was not available in the first version of GMID (v3.1); it is included in version 3.2. However, it requires a work-around to achieve the precision statistics on the 250 base pair fragment. The size standards can be overlayed from all samples in the run. The base pair sizes for the fragments can be displayed, the table exported to Microsoft Excel, and the Excel functions used to calculate the 250 base pair precision from a run.
Click here to view a screen capture of the size standard overlay with the sizing table beneath.)
GMID v3.2 added the ability to analyze Promega multiplex kits. It includes the addition of the penta nucleotide repeats and filtering specific to Y-STR kit data analysis.
