- Course Introduction
- 01: Evidence & DNA
- 02: Forensic Biology
- 03: DNA Extraction & Quantitation
- 04: DNA Amplification
- 05: Amplified DNA Product Separation
- 06: STR Data Analysis & Interpretation
- Data Troubleshooting
- Data Interpretation & Allele Calls
STaRCall™ is the software program that genotypes gel electrophoresis data for the Hitachi FMBIO® system. Sizes from the FMBIO® Analysis Software are imported into STaRCall, similar to the process used on GS/GT software on the CE platform. This program is similar in process to Genotyper®.
Allelic ladders are used to size the fragments from adjacent lanes; the closer the ladders are to the samples being run, the more precise the sizing. Where Genotyper® has a bin set associated with its macros, STaRCall™ has STR look-up tables maintained in Microsoft Excel. Optical density units are used to quantitate the fragments as the data is reviewed.
Click here to view the spreadsheet used to determine genotypes.
